SCIEX

Detection of a reduced monoclonal antibody (mAb) at low ng/ml concentration in biological samples by CESI-MS

Authors:              Stephen Lock and Richard Snell 2

Institutions:        Sciex, Warrington, UK & 2 GSK, Ware, UK

CE-MS especially techniques such as CESI (the integration of capillary electrophoresis (CE) and electrospray ionization (ESI) into a single process in a single device) are now enabling the easy connection of CE to a variety of mass spectrometers.

Current LCMS methodologies used to detect monoclonal antibody (mAb) in biological samples are focussed on targeted tryptic peptide quantitation which requires that the mAb undergo tryptic digestion before analysis.  The use of enzymatic digestion complicates the workflow and can affect the results obtained so there is an increasing interest in developing methods which can be used to detect either the intact antibody or larger antibody fragments which could simplify the overall workflow.

In this work we will describe how a CESI-MS method has been developed to quantitate a reduced monoclonal antibody in biological samples.  We will discuss the factors you need to consider when developing a CE-MS method to detect antibody fragments of 25 and 50kDa and show how injection and separation conditions can affect the sensitivity and peak resolution obtained.  We will demonstrate how using a surrogate mAb improves the reproducibility of electro-kinetic injections and highlight how CE-MS is now capable of detecting a mAb at low ng/ml sensitivities.

Important information about the workshops

Date: 24th September 2019

Time: Group 1     9.00 – 10.30

Group 2     11.00 – 12.30

Location: Intercollegiate Faculty of Biotechnology UG & MUG (Abrahama 58 Str., 80-307 Gdańsk)

Maximum number of participants: 15 (for each  group)

Cost: free

Please note that due to the limited number of spots, the order of registration to the course decides about the participation.

Introduction to R for bioanalysis

Authors:              Agnieszka Borsuk-De Moor

Institution:          Department of Biopharmaceutics and Pharmacodynamics, Faculty of Pharmacy

Medical University of Gdańsk; Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland

R is an open-source language and environment for statistical computing and graphics that has gained widespread acceptance in many branches such as biology, biotechnology, chemistry, and bioinformatics, becoming an almost indispensable tool.

During the workshop, participants will learn about R, RStudio, data processing, and visualisation, as well as data analysis in R.

The workshop will be divided into three parts:

  • Introduction to R (assignment operator, functions, objects, classes, data types, how to get help, and how to work with RStudio)
  • Introduction to data visualisation (how to create and modify plots with ggplot2 package)
  • Introduction to data analysis (how to describe data set through statistical parameters, exploratory data analysis, cluster analysis, PCA)

Important information about the workshops

Date: 24th September 2019

Time : 9.00 – 12.00

Location: Hotel Mercure Gdańsk Stare Miasto, Jana Heveliusza 22 Str.

Maximum number of participants: 40 (divided into two parallel groups)

Cost: free

Each participant is kindly asked to bring portable computer for the workshops.

Please note that due to the limited number of spots, the order of registration to the course decides about the participation.